Publications and Presentations

• Journal Publications:
• • Published several journal articles detailing experiments and analysis in physical, biological, and multidisciplinary research. Published experimental methods articles and a review of single molecule analysis techniques.

Peer Reviewed Journal Publications:

(in preparation, 2016) A. J. Manzo, H. Helvajian. A non intrusive, in situ process control tool for additive manufacturing that incorporates pulsed laser ultrasonic excitation with CW laser heterodyne detection.

(in preparation, 2016) A. J. Manzo, H. Helvajian. Enhanced mobility of a molecular absorbate induced via optically generated surface acoustic waves: Ethylene on crystalline silicon.

Shugaev, M. V., A. J. Manzo, C. Wu, V. Y. Zaitsev, H. Helvajian, and L. V. Zhigilei (2015) Strong enhancement of surface diffusion by nonlinear surface acoustic waves. Physical Review B, 91, 235450.
Manzo, A. J., A. O. Goushcha, N. M. Berezetska, V. N. Kharkyanen, and G. W. Scott (2011) Charge recombination time distributions in photosynthetic reaction centers exposed to alternating intervals of photoexcitation and dark relaxation. J. Phys. Chem. B 115:8534-8544.
Langelier, S.M.,E. Livak-Dahl, A. J. Manzo, B.N. Johnson, N.G. Walter, M.A. Burns (2011) Flexible casting of modular self-aligning microfluidic assembly blocks. Lab On A Chip 11:1679-1687.
Lund, K., A. J. Manzo, N. Dabby, N. Michelotti, A. Johnson-Buck, J. Nangreave, S. Taylor, R. Pei, M. N. Stojanovic, N. G. Walter, E. Winfree, and H. Yan (2010) Molecular Robots Guided by Prescriptive Landscapes. Nature 465:206-210.
Gérczei, T., B. N. Shah, A. J. Manzo, N. G. Walter, and C. C. Correll (2009) RNA Chaperones Stimulate Formation and Yield of the U3 snoRNA-Pre-rRNA Duplexes Needed for Eukaryotic Ribosome Biogenesis. J. Mol. Biol. 390:991-1006.
Manzo, A. J., A. O. Goushcha, Y. M. Barabash, V. N. Kharkyanen, and G. W. Scott (2009) Equilibration kinetics in isolated and membrane-bound photosynthetic reaction centers upon illumination: a method to determine the photoexcitation rate. Photosynth. Res. 101:35-45. (Erratum: (2010) Photosynth. Res. 103:65.)
Goushcha, A. O., A. J. Manzo, V. N. Kharkyanen, R. van Grondelle, and G. W. Scott (2004) Light induced equilibration kinetics in membrane bound photosynthetic reaction centers: Nonlinear dynamic effects in multiple scattering media. J. Phys. Chem. B, 108:2717-2725.
Goushcha, A. O., A. J. Manzo, G. W. Scott, L. N. Christophorov, P. P. Knox, Y. M. Barabash, M. T. Kapoustina, N. M. Berezetska, and V. N. Kharkyanen (2003) Self-Regulation Phenomena Applied to Bacterial Reaction Centers: 2. Nonequilibrium Adiabatic Potential: Dark and Light Conformations Revisited. Biophys. J. 84:1146-1160.

Review Articles:

Michelotti, N., A. Johnson-Buck, A. J. Manzo, and N. G. Walter (2012) Beyond DNA origami: the unfolding prospects of nucleic acid nanotechnology. WIREs: Nanomed Nanobiotechnol. 4:139-152.
Michelotti, N., C. de Silva, A. E. Johnson-Buck, A. J. Manzo, and N. G. Walter (2010) A bird’s eye view: Tracking slow nanometer-scale movements of single molecular nano-assemblies. Methods Enzymol. 475:121-148.
Walter, N. G., C. Y. Huang, A. J. Manzo, and M. A. Sobhy (2008) Do-it-yourself guide: how to use the modern single-molecule toolkit., Nat. Methods, 5:475-489.

Conference Proceedings:

Manzo, A. J., S. Kenderian, and H. Helvajian (2016) Application of Laser Ultrasonic Non Destructive Evaluation Technique to Additive Manufacturing Proc. SPIE xxxx.
Manzo, A. J., H. Helvajian (2014) Application of a laser heterodyne technique to characterize surface acoustic waves generated via a pulsed laser excitation. Proc. SPIE 8967, 89670N.
A. J. Manzo and H. Helvajian (2014) Demonstration of enhanced surface mobility of adsorbate cluster species by surface acoustic wave excitation induced by a pulsed laser, Proc. SPIE 8969, 896908.

Presentations and Posters:

University of Michigan, Ann Arbor
Manzo, A.J. (2011) Practical Considerations and Results of Real-Time Super-Resolution Imaging/Tracking of Single Deoxyribozyme Based Molecular Robots. An impromptu oral presentation at DNA 17: 17th International Conference on DNA Computing and Molecular Programming, California Institute of Technology, 9/19-23/2011, Pasadena, CA.
Manzo, A.J., A., S.K. Taylor, R. Pei, M.N. Stojanovic, N.G. Walter. (2011) Real-Time Super-Resolution Tracking of Single Deoxyribozyme Based Molecular Robots: Heterogeneities and effects of actuator multivalency and sequence recognition regions. A poster presentation at the 2011 Vaughan Symposium at the Chemistry Department, University of Michigan, 8/2011, Ann Arbor, MI.
Manzo, A.J., A., S.K. Taylor, R. Pei, M.N. Stojanovic, N.G. Walter. (2010) Real-Time Super-Resolution Tracking of Single Deoxyribozyme Based Molecular Robots. A poster presentation at the 2010 Vaughan Symposium at the Chemistry Department, University of Michigan, 8/18/2010, Ann Arbor, MI.
Manzo, A.J., A. Johnson-Buck, N.G. Walter, K. Lund, J. Nangreave, H. Yan, N. Michelotti, S.K. Taylor, R. Pei, M.N. Stojanovic, N. Dabby, E. Winfree (2010) Real-Time Super-Resolution Tracking of Single Deoxyribozyme Based Molecular Robots. An oral presentation at the First International Conference of RNA Nanotechnology and Therapeutics, 10/23-25/2010. Cleveland City Center, Cleveland, Ohio. Highlights of the conference: http://www.ncbi.nlm.nih.gov/pubmed/21604810
Manzo, A.J., A. Johnson-Buck, N.G. Walter, K. Lund, J. Nangreave, H. Yan, N. Michelotti, S.K. Taylor, R. Pei, M.N. Stojanovic, N. Dabby, E. Winfree (2010) Real-Time Super-Resolution Tracking of Single Deoxyribozyme Based Molecular Robots. An oral presentation at the 10th Annual Meeting of the Rustbelt RNA Meeting. 10/22-23/2010. Cleveland City Center, Cleveland, Ohio.
Manzo, A.J., A., S.K. Taylor, R. Pei, M.N. Stojanovic, N.G. Walter. (2010) Real-Time Super-Resolution Tracking of Single Deoxyribozyme Based Molecular Robots. A poster presentation at the Midwest Single Molecule Workshop at Washington University in Saint Louis, 7/26-27/2010. Washington University, Saint Louis, Missouri.
Manzo, A.J., E. livak-Dahl, M.D. Morris, M.A. Burns, and N.G. Walter. (2010). Characterization of the evanescent field produced by objective-based and prism-based total internal reflection fluorescence microcopy in PDMS channels and microfluidic assembly blocks. A poster presentation at the Microfluidics in Biomedical Sciences Training Program Symposium 2010. 5/19/2010. University of Michigan, Ann Arbor, Michigan.
Manzo, A.J., Lund, K., Dabby, N., Michelotti, N., Johnson-Buck, A., Nangreave, J., Taylor, S., Pei, R., Stojanovic, M.N., Winfree, E., Yan, H. and Walter, N.G.. (2010). Real-time super-resolution tracking of single deoxyribozyme based molecular robots. An oral presentation at the 11th Annual Meeting of the Michigan RNA Society. 3/27/2010. Michigan State University, East Lansing, Michigan.
Manzo, A.J., A., S.K. Taylor, R. Pei, M.N. Stojanovic, N.G. Walter. (2010) Real-Time Super-Resolution Tracking of Single Deoxyribozyme Based Molecular Robots. A poster presentation at the Molecular Biophysics Training Grant Symposium at The University of Michigan, 3/18/2010, Ann Arbor, MI.
Manzo, A.J., Chamaree de Silva, Nicole Michelotti, Alexander Johnson-Buck, Steven K. Taylor, Renjun Pei, Kyle Lund, Jeanette Nangreave, Hao Yan, Milan N. Stojanovic, Nils G. Walter. (2010). Real-time super-resolution tracking of single deoxyribozyme based molecular robots. A poster session at the Biophysical Society 54th Annual Meeting, 2/20-24/2010. San Francisco, California
Manzo, A.J., Chamaree de Silva, Nicole Michelotti, Alexander Johnson-Buck, Steven K. Taylor, Renjun Pei, Kyle Lund, Jeanette Nangreave, Hao Yan, Milan N. Stojanovic, Nils G. Walter. (2010). Real-time super-resolution tracking of single deoxyribozyme based molecular robots. A poster session at the Biophysical Society 54th Annual Meeting, 2/20-24/2010. San Francisco, California
Manzo, A.J., Lund, K., Dabby, N., Michelotti, N., Johnson-Buck, A., Nangreave, J., Taylor, S., Pei, R., Stojanovic, M.N., Winfree, E., Yan, H. and Walter, N.G. (2009). Real-time single particle tracking of deoxyribozyme based molecular robots. An oral presentation at the 10th Annual Meeting of the Michigan RNA Society. 4/11/2009. Albion College, Albion, Michigan.
Manzo, A.J., Lund, K., Dabby, N., Michelotti, N., Johnson-Buck, A., Nangreave, J., Taylor, S., Pei, R., Stojanovic, M.N., Winfree, E., Yan, H. and Walter, N.G.. (2010). Real-time single particle tracking of deoxyribozyme based molecular robots. A poster presentation at the Emergence in Chemical Systems Conference 2.0. 6/22-26/2009. University of Alaska, Anchorage, Alaska.
Manzo, A.J., C. de Silva, K. Lund, H. Yan, R. Pei, S.K. Taylor, M.N. Stojanovic, and N.G. Walter. (2008). Investigating the Behavior of Autonomously Moving Molecular Spiders On 2D Surfaces. A poster presentation at the University of Michigan PECRUM (Vaughan) Symposium. 8/01/08. Ann Arbor, Michigan.
Manzo, A.J., J.A. Hoerter, P. Zhu, M.D. Morris, and N.G. Walter. (2007). Nanometer-Scale Diffusive Motions of Surface-bound Fluorescent Probes as a Single Molecule Assay of Ribosome Function. A poster presentation at the Mechanics of Life Workshop. 6/11-13/2007. University of Michigan, Ann Arbor, Michigan.
Manzo, A.J., J.A. Hoerter, P. Zhu, M.D. Morris, and N.G. Walter. (2007). Nanometer-Scale Diffusive Motions of Surface-bound Fluorescent Probes as a Single Molecule Assay of Ribosome Function. A poster presentation at the 9th Annual Meeting of the Michigan RNA Society. 4/14/2007. University of Michigan, Ann Arbor, Michigan.
Manzo, A.J., C. de Silva, S.K. Taylor, M.N. Stojanovic, and N.G. Walter. (2006). Single Molecule Tracking of Autonomously Moving Molecular BioNanoassemblies: Spiders. A poster presentation at the Single Molecule Symposium at the University of Michigan, Ann Arbor “At the Single Molecule Frontier: Integration in Biology and Nanotechnology”. 5/18-20/06. Ann Arbor, Michigan.
Manzo, A.J., J.A. Hoerter, G. Kurtulus, P. Zhu, M.D. Morris, and N.G. Walter. (2006). Nanometer Scale Localization of Single Quantum Dots for Studies of Translation. A poster presentation at the 8th Annual Meeting of the Michigan RNA Society. 4/8/2006. Wayne State University, Detroit, Michigan. University of California, Riverside
Manzo, A.J., A.O. Goushcha, G.W. Scott, V.N. Kharkyanen, L.N. Christophorov, Y. Barabash, M.T. Kapoustina. (2005). Evidence for Slow Conformational Relaxation in Photosynthetic Reaction Centers. A poster session at the Biophysical Society 49th Annual Meeting, 2/12-16/2005. Long Beach, California
Manzo, A.J., A.O. Goushcha, and G.W. Scott. (2004). Non-equilibrium dynamic effects in isolated and membrane-bound reaction centers (RCs) from photosynthetic bacteria and the influence of multiple light-scattering effects in membrane-bound RCs. A poster presentation for the 24th Annual Conference on Statistical Physics of Macromolecules, sponsored by the Center for Nonlinear Studies at Los Alamos National Laboratory. 5/17-21/2004. Santa Fe, New Mexico.
Manzo, A.J., A.O. Goushcha, and G.W. Scott. (2004). Multiple light scattering effects and their manifestation in conformational memory effects of membrane-bound photosynthetic Reaction Centers. A poster presentation for the March Meeting of The American Physical Society. 3/22-26/2004. Montreal, Canada.
Manzo, A.J., A.O. Goushcha, and G.W. Scott. (2004). Multiple light scattering effects of membrane-bounds Rcs. A poster session at the 51st Annual Western Spectroscopy Association Conference. 1/28-30/2004. Asilomar Conference Center, Pacific Grove, CA
Manzo, A.J. (2004). Light-Induced Slow Structural Relaxation in Bacterial Photosynthetic Reaction Centers. A presentation for the Physical Chemistry Seminar. Chemistry Department, University of California, Riverside. 2/23/2004.
Manzo, A.J., A.O. Goushcha and G.W. Scott Multiple light scattering effects of membrane-bound RCs. (2003). Chemistry Department Annual Poster Session, University of California, Riverside. 9/26/2003
Manzo, A.J. (2003). Nonequilibrium dynamic structural transitions in bacterial photosynthetic reaction centers. A presentation for the Physical Chemistry Seminar. Chemistry Department, University of California, Riverside. 3/3/2003.
Manzo, A.J., A.O. Goushcha, G.W. Scott, V.N. Kharkyanen, L.N. Christophorov, Y. Barabash, M.T. Kapoustina. (2002). Evidence for a slow conformational relaxation in highly pre-illuminated reaction centers (RCs). A poster session at the Biophysical Society 46th Annual Meeting, 2/23-27/2002. San Francisco, California. Results abstracted in Biophysical Journal 82 (1): 1516 Part 2 JAN 2002).
Manzo, A.J., A.O. Goushcha, G.W. Scott. (2001). Relaxation kinetics using continuous wave and pulsed excitation in isolated bacterial photosynthetic reaction centers. American Physical Society California Section Inaugural Spring Meeting, 3/30 & 31/2001. University of California, Irvine Abstract available at (http://flux.aps.org/meetings/YR01/CSS01/abs/S150006.html)
Manzo, A.J., A.O. Goushcha and G.W. Scott. (2001). Relaxation kinetics using continuous wave and pulsed excitation in isolated bacterial photosynthetic reaction centers. A poster presented at the 48th Annual Western Spectroscopy Association Conference. 1/31-2/2/2001. Asilomar Conference Center, Pacific Grove, CA California State University, Northridge
Manzo, A.J., SP Lim, B.R. Cooper, D.L. Price. (1998). LMTO Calculations of the Magneto-Optics of Fully Relaxed FePt Multilayers. A presentation for the March Meeting of The American Physical Society. 3/16-20/1998. Los Angeles, CA. Abstract available at (http://flux.aps.org/meetings/YR98/BAPSMAR98/abs/S4790005.html)